rapid amplification cdna ends (race Search Results


smart rapid amplification cdna ends (race) cdna amplification  (Becton Dickinson)
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Becton Dickinson smart rapid amplification cdna ends (race) cdna amplification
Smart Rapid Amplification Cdna Ends (Race) Cdna Amplification, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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5’- 3’-race (rapid amplification cdna ends  (Becton Dickinson)
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Becton Dickinson 5’- 3’-race (rapid amplification cdna ends
5’ 3’ Race (Rapid Amplification Cdna Ends, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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5 -rapid amplification cdna ends (race) kit  (Becton Dickinson)
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Becton Dickinson 5 -rapid amplification cdna ends (race) kit
5 Rapid Amplification Cdna Ends (Race) Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rna ligase-mediated rapid amplification cdna ends (rlm–race  (Promega)
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Promega rna ligase-mediated rapid amplification cdna ends (rlm–race
Stimulation <t>of</t> <t>transcription</t> and expression of αE by transforming growth factor-β (TGF-β). (a) TK1 cells were treated for 16 hr with TGF-β and <t>RNA</t> was extracted at specific time-points and tested for αE transcripts. Probing for glyceraldehyde phosphate dehydrogenase (GAPDH) verified equal loading. (b) Cell-surface expression of αEβ7 by TK1 cells before (left panel) or after (right panel) treatment for 16 hr with TGF-β; black profiles show staining with monoclonal antibody (mAb) M290 (specific for αE), unfilled profiles show the negative control with isotyped-matched irrelevant mAb. (c) Nuclear run-on analysis to test for newly synthesized, nascent, αE transcripts in TK1 cells before and after treatment with TGF-β. 32P-labelled nuclear RNA was extracted from TK1 cells before or after overnight stimulation with TGF-β and from αE+ MTC-1 cells cultured in the presence of TGF-β. The RNA was tested for hybridization to αE cDNA. Detection of nascent mRNA for β-actin in all samples validated the technique.
Rna Ligase Mediated Rapid Amplification Cdna Ends (Rlm–Race, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rna ligase-mediated rapid amplification of cdna ends rlm–race  (Promega)
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Promega rna ligase-mediated rapid amplification of cdna ends rlm–race
Stimulation <t>of</t> <t>transcription</t> and expression of αE by transforming growth factor-β (TGF-β). (a) TK1 cells were treated for 16 hr with TGF-β and RNA was extracted at specific time-points and tested for αE transcripts. Probing for glyceraldehyde phosphate dehydrogenase (GAPDH) verified equal loading. (b) Cell-surface expression of αEβ7 by TK1 cells before (left panel) or after (right panel) treatment for 16 hr with TGF-β; black profiles show staining with monoclonal antibody (mAb) M290 (specific for αE), unfilled profiles show the negative control with isotyped-matched irrelevant mAb. (c) Nuclear run-on analysis to test for newly synthesized, nascent, αE transcripts in TK1 cells before and after treatment with TGF-β. 32P-labelled nuclear RNA was extracted from TK1 cells before or after overnight stimulation with TGF-β and from αE+ MTC-1 cells cultured in the presence of TGF-β. The RNA was tested for hybridization to αE <t>cDNA.</t> Detection of nascent mRNA for β-actin in all samples validated the technique.
Rna Ligase Mediated Rapid Amplification Of Cdna Ends Rlm–Race, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rapid+amplification+cdna+ends+(race/rna+ligase+mediated+rapid+amplification+of+cdna+ends+rlm+race/pmc01783235-147-12-15
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3′-rapid amplification of cdna ends (3′-race)  (Gallus BioPharmaceuticals)
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Gallus BioPharmaceuticals 3′-rapid amplification of cdna ends (3′-race)
Stimulation <t>of</t> <t>transcription</t> and expression of αE by transforming growth factor-β (TGF-β). (a) TK1 cells were treated for 16 hr with TGF-β and RNA was extracted at specific time-points and tested for αE transcripts. Probing for glyceraldehyde phosphate dehydrogenase (GAPDH) verified equal loading. (b) Cell-surface expression of αEβ7 by TK1 cells before (left panel) or after (right panel) treatment for 16 hr with TGF-β; black profiles show staining with monoclonal antibody (mAb) M290 (specific for αE), unfilled profiles show the negative control with isotyped-matched irrelevant mAb. (c) Nuclear run-on analysis to test for newly synthesized, nascent, αE transcripts in TK1 cells before and after treatment with TGF-β. 32P-labelled nuclear RNA was extracted from TK1 cells before or after overnight stimulation with TGF-β and from αE+ MTC-1 cells cultured in the presence of TGF-β. The RNA was tested for hybridization to αE <t>cDNA.</t> Detection of nascent mRNA for β-actin in all samples validated the technique.
3′ Rapid Amplification Of Cdna Ends (3′ Race), supplied by Gallus BioPharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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5′ rapid-amplification of cdna ends (race)  (BGI Shenzhen)
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BGI Shenzhen 5′ rapid-amplification of cdna ends (race)
Stimulation <t>of</t> <t>transcription</t> and expression of αE by transforming growth factor-β (TGF-β). (a) TK1 cells were treated for 16 hr with TGF-β and RNA was extracted at specific time-points and tested for αE transcripts. Probing for glyceraldehyde phosphate dehydrogenase (GAPDH) verified equal loading. (b) Cell-surface expression of αEβ7 by TK1 cells before (left panel) or after (right panel) treatment for 16 hr with TGF-β; black profiles show staining with monoclonal antibody (mAb) M290 (specific for αE), unfilled profiles show the negative control with isotyped-matched irrelevant mAb. (c) Nuclear run-on analysis to test for newly synthesized, nascent, αE transcripts in TK1 cells before and after treatment with TGF-β. 32P-labelled nuclear RNA was extracted from TK1 cells before or after overnight stimulation with TGF-β and from αE+ MTC-1 cells cultured in the presence of TGF-β. The RNA was tested for hybridization to αE <t>cDNA.</t> Detection of nascent mRNA for β-actin in all samples validated the technique.
5′ Rapid Amplification Of Cdna Ends (Race), supplied by BGI Shenzhen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3′ rapid amplification of cdna ends (race)  (BEI Resources)
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BEI Resources 3′ rapid amplification of cdna ends (race)
Stimulation <t>of</t> <t>transcription</t> and expression of αE by transforming growth factor-β (TGF-β). (a) TK1 cells were treated for 16 hr with TGF-β and RNA was extracted at specific time-points and tested for αE transcripts. Probing for glyceraldehyde phosphate dehydrogenase (GAPDH) verified equal loading. (b) Cell-surface expression of αEβ7 by TK1 cells before (left panel) or after (right panel) treatment for 16 hr with TGF-β; black profiles show staining with monoclonal antibody (mAb) M290 (specific for αE), unfilled profiles show the negative control with isotyped-matched irrelevant mAb. (c) Nuclear run-on analysis to test for newly synthesized, nascent, αE transcripts in TK1 cells before and after treatment with TGF-β. 32P-labelled nuclear RNA was extracted from TK1 cells before or after overnight stimulation with TGF-β and from αE+ MTC-1 cells cultured in the presence of TGF-β. The RNA was tested for hybridization to αE <t>cDNA.</t> Detection of nascent mRNA for β-actin in all samples validated the technique.
3′ Rapid Amplification Of Cdna Ends (Race), supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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5′ 3′ rapid amplification cdna ends  (Becton Dickinson)
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Becton Dickinson 5′ 3′ rapid amplification cdna ends
Stimulation <t>of</t> <t>transcription</t> and expression of αE by transforming growth factor-β (TGF-β). (a) TK1 cells were treated for 16 hr with TGF-β and RNA was extracted at specific time-points and tested for αE transcripts. Probing for glyceraldehyde phosphate dehydrogenase (GAPDH) verified equal loading. (b) Cell-surface expression of αEβ7 by TK1 cells before (left panel) or after (right panel) treatment for 16 hr with TGF-β; black profiles show staining with monoclonal antibody (mAb) M290 (specific for αE), unfilled profiles show the negative control with isotyped-matched irrelevant mAb. (c) Nuclear run-on analysis to test for newly synthesized, nascent, αE transcripts in TK1 cells before and after treatment with TGF-β. 32P-labelled nuclear RNA was extracted from TK1 cells before or after overnight stimulation with TGF-β and from αE+ MTC-1 cells cultured in the presence of TGF-β. The RNA was tested for hybridization to αE <t>cDNA.</t> Detection of nascent mRNA for β-actin in all samples validated the technique.
5′ 3′ Rapid Amplification Cdna Ends, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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5*- and 3*-rapid amplification of cdna ends (race)  (Schuell GmbH)
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Schuell GmbH 5*- and 3*-rapid amplification of cdna ends (race)
Stimulation <t>of</t> <t>transcription</t> and expression of αE by transforming growth factor-β (TGF-β). (a) TK1 cells were treated for 16 hr with TGF-β and RNA was extracted at specific time-points and tested for αE transcripts. Probing for glyceraldehyde phosphate dehydrogenase (GAPDH) verified equal loading. (b) Cell-surface expression of αEβ7 by TK1 cells before (left panel) or after (right panel) treatment for 16 hr with TGF-β; black profiles show staining with monoclonal antibody (mAb) M290 (specific for αE), unfilled profiles show the negative control with isotyped-matched irrelevant mAb. (c) Nuclear run-on analysis to test for newly synthesized, nascent, αE transcripts in TK1 cells before and after treatment with TGF-β. 32P-labelled nuclear RNA was extracted from TK1 cells before or after overnight stimulation with TGF-β and from αE+ MTC-1 cells cultured in the presence of TGF-β. The RNA was tested for hybridization to αE <t>cDNA.</t> Detection of nascent mRNA for β-actin in all samples validated the technique.
5* And 3* Rapid Amplification Of Cdna Ends (Race), supplied by Schuell GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rapid amplification of cdna ends-pcr (race-pcr)  (Becton Dickinson)
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Becton Dickinson rapid amplification of cdna ends-pcr (race-pcr)
Stimulation <t>of</t> <t>transcription</t> and expression of αE by transforming growth factor-β (TGF-β). (a) TK1 cells were treated for 16 hr with TGF-β and RNA was extracted at specific time-points and tested for αE transcripts. Probing for glyceraldehyde phosphate dehydrogenase (GAPDH) verified equal loading. (b) Cell-surface expression of αEβ7 by TK1 cells before (left panel) or after (right panel) treatment for 16 hr with TGF-β; black profiles show staining with monoclonal antibody (mAb) M290 (specific for αE), unfilled profiles show the negative control with isotyped-matched irrelevant mAb. (c) Nuclear run-on analysis to test for newly synthesized, nascent, αE transcripts in TK1 cells before and after treatment with TGF-β. 32P-labelled nuclear RNA was extracted from TK1 cells before or after overnight stimulation with TGF-β and from αE+ MTC-1 cells cultured in the presence of TGF-β. The RNA was tested for hybridization to αE <t>cDNA.</t> Detection of nascent mRNA for β-actin in all samples validated the technique.
Rapid Amplification Of Cdna Ends Pcr (Race Pcr), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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50 rapid amplification cdna ends (50 race  (Becton Dickinson)
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Becton Dickinson 50 rapid amplification cdna ends (50 race
Stimulation <t>of</t> <t>transcription</t> and expression of αE by transforming growth factor-β (TGF-β). (a) TK1 cells were treated for 16 hr with TGF-β and RNA was extracted at specific time-points and tested for αE transcripts. Probing for glyceraldehyde phosphate dehydrogenase (GAPDH) verified equal loading. (b) Cell-surface expression of αEβ7 by TK1 cells before (left panel) or after (right panel) treatment for 16 hr with TGF-β; black profiles show staining with monoclonal antibody (mAb) M290 (specific for αE), unfilled profiles show the negative control with isotyped-matched irrelevant mAb. (c) Nuclear run-on analysis to test for newly synthesized, nascent, αE transcripts in TK1 cells before and after treatment with TGF-β. 32P-labelled nuclear RNA was extracted from TK1 cells before or after overnight stimulation with TGF-β and from αE+ MTC-1 cells cultured in the presence of TGF-β. The RNA was tested for hybridization to αE <t>cDNA.</t> Detection of nascent mRNA for β-actin in all samples validated the technique.
50 Rapid Amplification Cdna Ends (50 Race, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Stimulation of transcription and expression of αE by transforming growth factor-β (TGF-β). (a) TK1 cells were treated for 16 hr with TGF-β and RNA was extracted at specific time-points and tested for αE transcripts. Probing for glyceraldehyde phosphate dehydrogenase (GAPDH) verified equal loading. (b) Cell-surface expression of αEβ7 by TK1 cells before (left panel) or after (right panel) treatment for 16 hr with TGF-β; black profiles show staining with monoclonal antibody (mAb) M290 (specific for αE), unfilled profiles show the negative control with isotyped-matched irrelevant mAb. (c) Nuclear run-on analysis to test for newly synthesized, nascent, αE transcripts in TK1 cells before and after treatment with TGF-β. 32P-labelled nuclear RNA was extracted from TK1 cells before or after overnight stimulation with TGF-β and from αE+ MTC-1 cells cultured in the presence of TGF-β. The RNA was tested for hybridization to αE cDNA. Detection of nascent mRNA for β-actin in all samples validated the technique.

Journal:

Article Title: Studies on transcriptional regulation of the mucosal T-cell integrin ?E?7 (CD103)

doi: 10.1046/j.1365-2567.2001.01232.x

Figure Lengend Snippet: Stimulation of transcription and expression of αE by transforming growth factor-β (TGF-β). (a) TK1 cells were treated for 16 hr with TGF-β and RNA was extracted at specific time-points and tested for αE transcripts. Probing for glyceraldehyde phosphate dehydrogenase (GAPDH) verified equal loading. (b) Cell-surface expression of αEβ7 by TK1 cells before (left panel) or after (right panel) treatment for 16 hr with TGF-β; black profiles show staining with monoclonal antibody (mAb) M290 (specific for αE), unfilled profiles show the negative control with isotyped-matched irrelevant mAb. (c) Nuclear run-on analysis to test for newly synthesized, nascent, αE transcripts in TK1 cells before and after treatment with TGF-β. 32P-labelled nuclear RNA was extracted from TK1 cells before or after overnight stimulation with TGF-β and from αE+ MTC-1 cells cultured in the presence of TGF-β. The RNA was tested for hybridization to αE cDNA. Detection of nascent mRNA for β-actin in all samples validated the technique.

Article Snippet: The transcription start sites were identified by RNA Ligase-Mediated Rapid Amplification of cDNA Ends (RLM–RACE; Promega UK Ltd, Southampton, UK), according to the supplier's protocol.

Techniques: Expressing, Staining, Negative Control, Synthesized, Cell Culture, Hybridization

Stimulation of transcription and expression of αE by transforming growth factor-β (TGF-β). (a) TK1 cells were treated for 16 hr with TGF-β and RNA was extracted at specific time-points and tested for αE transcripts. Probing for glyceraldehyde phosphate dehydrogenase (GAPDH) verified equal loading. (b) Cell-surface expression of αEβ7 by TK1 cells before (left panel) or after (right panel) treatment for 16 hr with TGF-β; black profiles show staining with monoclonal antibody (mAb) M290 (specific for αE), unfilled profiles show the negative control with isotyped-matched irrelevant mAb. (c) Nuclear run-on analysis to test for newly synthesized, nascent, αE transcripts in TK1 cells before and after treatment with TGF-β. 32P-labelled nuclear RNA was extracted from TK1 cells before or after overnight stimulation with TGF-β and from αE+ MTC-1 cells cultured in the presence of TGF-β. The RNA was tested for hybridization to αE cDNA. Detection of nascent mRNA for β-actin in all samples validated the technique.

Journal:

Article Title: Studies on transcriptional regulation of the mucosal T-cell integrin ?E?7 (CD103)

doi: 10.1046/j.1365-2567.2001.01232.x

Figure Lengend Snippet: Stimulation of transcription and expression of αE by transforming growth factor-β (TGF-β). (a) TK1 cells were treated for 16 hr with TGF-β and RNA was extracted at specific time-points and tested for αE transcripts. Probing for glyceraldehyde phosphate dehydrogenase (GAPDH) verified equal loading. (b) Cell-surface expression of αEβ7 by TK1 cells before (left panel) or after (right panel) treatment for 16 hr with TGF-β; black profiles show staining with monoclonal antibody (mAb) M290 (specific for αE), unfilled profiles show the negative control with isotyped-matched irrelevant mAb. (c) Nuclear run-on analysis to test for newly synthesized, nascent, αE transcripts in TK1 cells before and after treatment with TGF-β. 32P-labelled nuclear RNA was extracted from TK1 cells before or after overnight stimulation with TGF-β and from αE+ MTC-1 cells cultured in the presence of TGF-β. The RNA was tested for hybridization to αE cDNA. Detection of nascent mRNA for β-actin in all samples validated the technique.

Article Snippet: The transcription start sites were identified by RNA Ligase-Mediated Rapid Amplification of cDNA Ends (RLM–RACE; Promega UK Ltd, Southampton, UK), according to the supplier's protocol.

Techniques: Expressing, Staining, Negative Control, Synthesized, Cell Culture, Hybridization